This study explores the use of three-dimensional (3D) printing technology to create polylactic acid (PLA) models of human femoral diaphyses for biomechanical testing. By optimizing printing parameters such as infill density, wall layers, and resolution, the research successfully emulates the flexural biomechanical response of normal human femur bones. The findings demonstrate that these cost-effective and reproducible 3D-printed models could serve as valuable resources for surgeons and researchers, providing a promising alternative for biomechanical studies.